Figure 2
From: MDM4 inhibition: a novel therapeutic strategy to reactivate p53 in hepatoblastoma
MDM4 inhibition with ATSP-7041 and NSC207895 lead to apoptosis of HB cell lines. (a) HepG2, Huh-6, HepT1, and B6-2 cells were exposed to varying indicated concentrations of ATSP-7041 for 48 h. MTT assays were performed at 48 h to assess viability. Error bars represent SD. Data shown are representative of at least three independent experiments performed with three replicate wells each time. (b) HepG2, Huh-6, HepT1, and B6-2 cells were exposed to varying indicated concentrations of NSC207895 for 48 h. MTT assays were performed at 48 h to asses viability. Error bars represent SD. Data shown are representative of at least three independent experiments performed with three replicate wells each time. (c) HepG2, Huh-6, HepT1, and B6-2 cells were exposed to varying indicated concentrations of Nutlin-3a for 48 h. MTT assays were performed at 48 h to asses viability. Error bars represent SD. Data shown are representative of at least three independent experiments performed with three replicate wells each time. (d) IC50 values determined in the assays shown in (a–c). (e) Protein lysis from cells treated with 10 μM ATSP-7041 for 24 h was compared to that from untreated cells (0 h). Immunoblotting for total PARP, PARP cleavage, and Caspase-3 cleavage was done to assess apoptosis. β-Actin immunoblotting was used as a loading control. Date shown are representative of at least three independent experiments. (f) Protein lysis from cells treated with 10 μM NSC207895 for 4 or 8 h was compared to that from untreated cells (0 h). Immunoblotting for total PARP, PARP cleavage, and Caspase-3 cleavage was done to assess apoptosis. β-Actin immunoblotting was used as a loading control. Data shown are representative of at least three independent experiments. Full length blots for data shown in e and f are presented in Supplementary Fig. 7.
