Table 4 Clinical features of CLL samples analysed by data dependant acquisition (DDA) to generate a CLL-specific spectral library for mapping data acquired by SWATH-MS.

From: Assessing technical and biological variation in SWATH-MS-based proteomic analysis of chronic lymphocytic leukaemia cells

Sample

Gender

IGHV %

Chromosomal abnormalities

Prior therapy (Y/N)

WBC (109/l)

Lymphocyte count (109/l)

% viable cells after thaw

CLL-1

F

5.9

13q−

N

63.5

63.4

88

CLL-2

M

IGHV failed to identify single dominant clone 5 different clones identified 0–12

13q−, 11q−

N

21.7

15.5

90

CLL-3

M

NA

17p−

Y

48.1

45

78

CLL-4

M

NA

13q−

Y

24.1

21.9

87

CLL-5

M

2.08

13q−

N

144.3

137

80

CLL-6

M

NA

17p−, 13q−

Y

49.2

44.7

82

CLL-7

M

0

Normal

Y

65.4

63.3

84

CLL-8

M

NA

13q−

Y

110.2

102.9

90

CLL-9

F

0

Normal

Y

52.7

46.7

96

CLL-10

F

NA

13q−

N

149.9

144.8

88

CLL-11

F

0

Normal

Y

31.3

27.9

92

CLL-12

M

0

17p−

Y

291.6

NA

70

CLL-13

M

NA

17p−, 13q−

Y

246.4

NA

77

CLL-14

F

5.9

11q−, 12+

Y

19.3

17.4

90

  1. Prior therapy consisted of various combinations of glucocorticoid, chlorambucil, fludarabine, or fludarabine plus cyclophosphamide. IGHV refers to somatic mutation in the IGHV gene of CLL cells compared with the gene sequence of the nearest germ line, where < 2% was classed as UM-CLL and > 2% was classed as M-CLL. CLL samples were tested by interphase fluorescence in situ hybridization for del17p13 (17p−), del11q23 (11q−), trisomy 12 (12+), and del13q14 (13q−). 17p and 11q− are regarded as high-risk chromosomal abnormalities.