Figure 2
From: Temperature and species-dependent regulation of browning in retrobulbar fat
Transcriptomic analysis of murine fat depots reveals significant similarity between orbital fat and interscapular brown fat. (A) Principal component analysis of transcriptome data from mouse brown fat (BF), epididymal fat (EF), inguinal fat (IF) and orbital fat (OF) demonstrates that BF and OF segregate more closely to each other than to EF and IF. (B) Sample-wise correlation of transcriptome data from BF, EF, IF and OF demonstrates that transcriptome of OF is more closely correlated with that of BF than with the transcriptomes of EF and IF. (C) Heat map of transcriptome data from mouse BF, EF, IF and OF demonstrates more significant shared gene expression between OF and BF. There is less significant overlap between OF and either EF or IF. Heatmap was created using Matlab 2018. (D) qPCR validates transcriptome data, demonstrating that Cxcl12 transcript variant 2 is enriched in orbital fat (OF) compared to brown fat (BF), epididymal fat (EF), and inguinal fat (IF). ** indicates p < 0.0001 by one-way Anova. (E) qPCR demonstrates that brown fat markers Ucp1, Cidea, and Gdf5 are enriched in murine BF and OF compared to EF and IF. ** indicates p < 0.0001 by one-way Anova. * indicates p = 0.006 by one-way Anova.
