Figure 4

Mouse orbital fat resembles interscapular brown fat after thermoneutral adaptation. (A) H&E section of interscapular brown fat (BF) from CD1 mice housed at 30 °C for 4 weeks demonstrates increased proportion of adipocytes with large lipid droplets characteristic of white adipose tissue. (B) H&E section of orbital fat (OF) from CD1 mouse housed at 30 °C for 4 weeks demonstrates increased proportion of adipocytes with large lipid droplets characteristic of white adipose tissue. (C) H&E section of OF from C57BL/6 mouse housed at 30 °C for 4 weeks demonstrates increased proportion of adipocytes with large lipid droplets characteristic of white adipose tissue. (D-I) smFISH demonstrates decreased Ucp1 expression in interscapular brown fat of mice adapted to thermoneutral temperatures (30 °C) compared to those housed near room temperature (22 °C). E Higher magnification of D. F Higher magnification of E. (H) Higher magnification of G. (I) Higher magnification of H. (J-O) smFISH demonstrates decreased Ucp1 expression in orbital fat of mice adapted to thermoneutral temperatures (30 °C) compared to those housed near room temperature (22 °C). (K) Higher magnification of J. (L) Higher magnification of K. (N) Higher magnification of M. O Higher magnification of N. (P-U) SmfISH demonstrates decreased Gdf5 and Cxcl12 expression in orbital fat of mice adapted to thermoneutral temperatures (30 °C) compared to those housed near room temperature (22 °C). Q Higher magnification of P. (R) Higher magnification of Q. (T) Higher magnification of S. (U) Higher magnification of T. Scale bars = 50 μm.