Figure 4

Combination of NLRC5 expression and load of mutation or neoantigen provide prognostic information. (a) Kaplan–Meier estimates of five year overall survival of patients with high and low mutation load (Left), NLRC5 gene expression (Middle), and NLRC5 methylation (Right). Patients in the TCGA melanoma cohort were stratified by medians into high and low groups (n = 159 and n = 160). (b) Kaplan–Meier estimates of five year overall survival of patients with varying levels of two factors, NLRC5 expression and mutation load (Left) and NLRC5 methylation and mutation load (Right). Patients were stratified by two factors (NLRC5 expression/NLRC5 methylation and mutation load) in a similar fashion with (a), yielding four groups (high NLRC5 expression/NLRC5 methylation and high mutation load, likewise, high and low, low and high, low and low). Pairwise log-rank test was used to analyze the survival in indicated pairs. Hazard ratio (HR) and 95% confidence interval (CI) was determined by multivariate analysis using Cox regression model (see Methods). *p < 0.05; **p < 0.01; ***p < 0.001.