Figure 2
From: Role of PDZ-binding motif from West Nile virus NS5 protein on viral replication
PDZome binding profiles of WNV PBM by two high-throughput techniques: holdup assay (A) and Split-Nanoluciferase Protein Complementation Assay (N2H) (B). (A) Binding intensity profiles for the PDZome. PDZ domains revealed by holdup assay are ranked on the basis of the BIs of WNV NS5 PBM. Zoomed-in view (corresponding to the red box) shows the 29 PDZ NS5 binders which displayed significant BI values higher than 0.2. B. Frequency distribution for NLR(NS5) – NLR(NS5∆PBM) obtained by N2H method are ranked on their values. Zoomed-in view (corresponding to the red box) shows 28 PDZ NS5 binders displayed an NLR difference > 0.25 NLR.
