Figure 1 | Scientific Reports

Figure 1

From: Development of event-specific detection method for identification of insect resistant NIBGE-1601 cotton harboring double gene Cry1Ac-Cry2Ab construct

Figure 1

PCR for transgene analysis of NIBGE-1601 cotton event using SadI, nptII and Cry1Ac specific primers. 1 = NIBGE-1601 cotton, 2 = Triple gene (TG) NIBGE cotton (P1), 3 = non-GM Coker (P2), 4 = TG NIBGE cotton (P2), 5 = Bollgard-II, 6 = negative control (water), 7 = NIBGE-1601 cotton, 8 = TG NIBGE cotton (P1), 9 = non-GM Coker (P2), 10 = TG NIBGE cotton (P2), 11 = Bollgard-II, 12 = negative control (water), 13 = NIBGE-1601 cotton, 14 = TG NIBGE cotton (P1), 15 = non-GM Coker (P2), 16 = TG NIBGE cotton (P2), 17 = Bollgard-II, 18 = negative control (water)* Primers: S1F + S2R (Sad1; cotton fiber gene specific, 107 bp), APH2 Short + APH2 Reverse (nptII, 215 bp), CR1BDF5 + CR1BDR5 (NIBGE Cry1Ac gene specific, 521 bp).

Back to article page