Table 2 Transgene Analysis of Insect Resistant NIBGE-1601 Cotton Event.

From: Development of event-specific detection method for identification of insect resistant NIBGE-1601 cotton harboring double gene Cry1Ac-Cry2Ab construct

Samples & Tests

PCR using specific primers (DNA based test)

SadI

nptII

NIBGE Cry1Ac

NIBGE Cry2Ab

NIBGE EPSPS

NIBGE-1601 event specific primers

Mon 531 (BG)

Mon 15,985 (BGII)

Mon 1445 (RR)

NIBGE-1601 cotton

 + 

 + 

 + 

 + 

 − 

 + 

 − 

 − 

 − 

Coker-312 cotton control (-ve)

 + 

 − 

 − 

 − 

 − 

 − 

 − 

 − 

 − 

BGII + RR MON cotton control

 + 

 + 

 − 

 − 

 − 

 − 

 + 

 + 

 + 

  1.  − band absent or no amplification, + band present or amplification.