Figure 4
From: Enhancement of elastin expression by transdermal administration of sialidase isozyme Neu2
Enhancement of elastin expression by mammalian sialidase Neu2. (A–D) Expression of various sialidase isozyme mRNAs in adipose tissue (n = 6 in each) and tissues including the dermis and muscle (n = 5 in each). *P < 0.05, ***P < 0.001 (unpaired t-test). (E) Neu2 expression in lateral abdomen tissue was determined by immunohistochemical staining. e, epithelium; d, dermis; m, muscle; sf, subcutaneous fat; sg, sebaceous glands. Scale bar, 1 mm. (F,G) Elastin (n = 8 in each) and elastin mRNA levels (n = 6–7) in the rat skin were measured after applying PBS, CAGE or Neu2 in CAGE or after applying Neu2 first and then CAGE twice a day for 5 days. ***P < 0.001 vs. PBS; ††P < 0.01, †††P < 0.001 vs. CAGE, ‡‡‡P < 0.001 vs. Neu2 in CAGE (one-way ANOVA with Bonferroni's multiple comparison test).
