Table 2 Formulation and proximate composition of the experimental diets (% dry matter basis).

From: The protective role of daidzein in intestinal health of turbot (Scophthalmus maximus L.) fed soybean meal-based diets

Ingredients

FM

SBM

DAID

Fish meala

68

40.80

40.80

Soybean mealb

0

37.90

37.90

α-Starch

16

11.55

11.546

Fish oil

4.80

6.70

6.70

Soy lecithin

0.50

0.50

0.50

Vitamin premixc

1

1

1

Mineral premixd

0.50

0.50

0.50

Choline chloride

0.30

0.30

0.30

Monocalcium phosphate

0.50

0.50

0.50

Ethoxyquin

0.05

0.05

0.05

Yttrium oxide

0.10

0.10

0.10

Calcium propionate

0.10

0.10

0.10

Cellulose

8.15

0

0

Daidzeine

0

0

0.004

Sum

100

100

100

Proximate composition

Dry matter content

97.07

97.11

97.75

Crude protein

50.41

50.15

51.46

Crude lipid

9.52

10.29

10.10

Content of daidzein (μg/g)

Nd

35.89

62.87

  1. FM fish meal diet, SBM soybean meal diet, DAID 40 mg/kg daidzein included into SBM diet, Nd no detected.
  2. a,bPurchased from Qingdao Seven Great Bio-tech Company Limited (Qingdao, China), Fish meal: crude protein, 74.04%; crude lipid, 9.97%; Soybean meal: crude protein, 53.12%; crude lipid: 2.12%.
  3. cVitamin mixture (mg kg−1): VA, 32; VB1, 25; VB2, 45; VB6, 20; VB12, 10; Niacinaminde, 200; Inositol, 800; Calcium pantothenate, 60; VH, 60; Folate, 20; VE, 240; VK, 10; VC phosphate, 2000; VD, 5; Antioxidant, 3; Microcrystalline cellulose, 6470.
  4. dMineral mixture (mg kg−1): Mg, 313; Fe, 79.10; Zn, 62.60; Mn, 46.60; I, 2; Se, 0.90; Cu, 6.40; Zeolite powder, 3485.
  5. ePurchased from Shanghai Macklin Biochemical Technology Co., Ltd., the purity was more than 98.051%.