Table 1 Effect of different production replicates and muscle types on proteolysis and apoptosis features of porcine skeletal muscles over 7-day postmortem aging.

Parameters^a,b,c	Replicate (R)^d		Muscle (M)^e		Aging (A)		S.E.M^f	P-value
Parameters^a,b,c	Apr–Sep	July–Jan	LD	PM	1 day	7 days	S.E.M^f	R	M	A
MMP	0.082	0.092	0.092	0.081	0.094	0.079	0.002	< .001	< .001	< .0001
Cytochrom c	1.76	1.91	0.79	2.88	1.99	1.68	0.22	0.20	< .0001	0.003
HSP27	0.90	0.78	0.61	1.07	0.98	0.70	0.08	0.018	< .0001	< .0001
Αβ-crystallin	1.75	1.42	1.39	1.78	1.74	1.43	0.30	0.071	0.029	0.092
Calpain-1 80 kDa	0.16	0.20	0.16	0.20	0.19	0.17	0.02	0.002	0.004	0.025
Calpain-1 78 kDa	0.38	0.41	0.35	0.44	0.41	0.38	0.02	0.054	< .0001	0.050
Calpain-1 76 kDa	0.46	0.39	0.49	0.36	0.40	0.45	0.03	0.001	< .0001	0.005
Troponin T intact	1.72	1.73	2.14	1.32	2.71	0.75	0.48	0.98	0.071	0.002
Troponin T Degradation 35 kDa	1.04	0.68	0.95	0.78	0.44	1.29	0.19	0.038	0.17	< .001
Troponin T Degradation 30 kDa	0.92	0.43	1.05	0.30	0.32	1.03	0.40	0.10	0.039	0.037
Desmin intact	1.21	1.59	1.22	1.58	1.58	1.22	0.17	0.002	0.094	< .001
Desmin degradation 39 kDa	1.02	1.21	0.99	1.24	1.08	1.15	0.12	0.085	0.046	0.22

^aMMP (mitochondrial membrane permeability) was obtained by measuring UV absorption peak at 540 nm of the isolated mitochondria suspensions, higher number indicates lower MMP.
^bProtein abundance except calpain-1 was expressed as relative ratio of band intensity compared to the corresponding bands of the reference samples.
^cThree bands of 80, 78, and 76 kDa of calpain-1 were quantified and expressed as the ratio of each band that relative to the total intensity.
^dReplicate: July–Jan, pigs weaned in July 2016 and harvested in January 2017; Apr–Sep: pigs weaned in April 2017 and harvested in September 2017.
^eMuscle: LD, longissimus dorsi; PM, psoas major.
^fPooled standard errors of means.

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