Figure 1

Synergistic effects of BKM120 and olaparib on the growth of TNBC cells. (A) IC₅₀ values for the effects of BKM120 and olaparib on the viability of HCC1937, MDA-MB-436, MDA231-LM2 and MDA-MB-231 cells measured by MTT assay. CI curves for concurrent treatment with BKM120 and olaparib in (B) MDA-MB-231 cells, (C) MDA-MB-436 cells, (D) HCC1937 cells and (E) MDA231-LM2 cells. CI < 1.0 indicated a synergistic effect. The combination ratios of BKM120/olaparib are 2:1, 1:1, 1:2, 1:4, 1:6 and 1:8, respectively. (F) The combined effects of BKM120 and olaparib on the proliferation of MDA-MB-231 cells measured by colony-forming assay. (G) The combined effects of BKM120 and olaparib on the apoptosis of MDA-MB-231 cells. Apoptotic cells measured by Annexin V-FITC and PI double staining. Q₁ and Q₄ respectively represent the proportions of death cells and living cells, Q₂ and Q₃ were used to calculate apoptotic cells. Figures show a representative experiment of three independent experiments. Error bars represent SEM from the mean of three separate experiments. ^*P < 0.05 and ^**P < 0.01 compared to control. ^#P < 0.05 and ^##P < 0.01 compared to 3.2 μM BMK120-treated group.