Figure 2 | Scientific Reports

Figure 2

From: BKM120 sensitizes BRCA-proficient triple negative breast cancer cells to olaparib through regulating FOXM1 and Exo1 expression

Figure 2

Effects of BKM120 and olaparib on DNA damage in MDA-MB-231 cells. (A) DNA damage was assessed by alkaline comet assay. The tail DNA percentage of comet assay at indicated concentration of BKM120, olaparib, and their combination. (B) Effects of drugs on cellular ROS analyzed by flow cytometry. (C) RT-PCR analysis of the expression of HMOX-1 induced by drugs. (D) Percentage of relative intensity obtained from the corresponding RT-PCR. (E) Effects of drugs on the expressions of pADPr, PARP1 and PARP2 analyzed by western blots. (F) Relative intensity expression obtained from the corresponding western blots. (G) Effects of drugs on the expressions of PARP1 and PARP2 in MDA-MB-231 cells analyzed by RT-PCR. (H) Relative intensity expression obtained from the corresponding RT-PCR. Error bars represent SEM from the mean of three separate experiments. *P < 0.05 and **P < 0.01 compared to control, #P < 0.05 and ##P < 0.01 compared to 3.2 μM BMK120-treated group.

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