Figure 4

Effects of BKM120 and olaparib on the repair factors of DNA DSBs. Effects of BKM120, olaparib, and a combination of BKM120 with olaparib on the expressions of BRCA1, BRCA2, Rad51 and Rad52 in MDA-MB-231 cells analyzed by (A) RT-PCR and (B) western blots. Relative intensity expression obtained from the corresponding (C) RT-PCR and (D) western blots. Error bars represent SEM from the mean of three separate experiments. *P < 0.05 and **P < 0.01 compared to control, #P < 0.05 and ##P < 0.01 compared to 3.2 μM BMK120-treated group.