Figure 7 | Scientific Reports

Figure 7

From: BKM120 sensitizes BRCA-proficient triple negative breast cancer cells to olaparib through regulating FOXM1 and Exo1 expression

Figure 7

The roles of FOXM1 and Exo1 in the repair of DNA DSBs. (A) The expression of BRCA1, BRCA2, Rad51 and Rad52 after silence the expression of FOXM1 and Exo1 analyzed by western blots. (B) Relative intensity expression obtained from the corresponding western blots. (C) Comet assay analysis of DNA damage after silence the expression of FOXM1 and Exo1. (D) Apoptosis of MDA-MB-231 cells measured by Annexin V-FITC and PI double staining after silence the expression of FOXM1 and Exo1. (E) Immunofluorescence analysis of γh2AX after silence the expression of FOXM1 and Exo1. Error bars represent SEM from the mean of three separate experiments. *P < 0.05 and **P < 0.01 represent FOXM1 siRNA group compared to NC siRNA group, #P < 0.05 and ##P < 0.01 represent Exo1 siRNA group compared to NC siRNA group.

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