Table 2 Primer sequences for the apoptosis factors in RT-PCR.

From: BKM120 sensitizes BRCA-proficient triple negative breast cancer cells to olaparib through regulating FOXM1 and Exo1 expression

Name

Sense (5′-3′)

Antisense (5′-3′)

HMOX1

CCTAAACTTCAGAGGGGGCG

ATGGCTCAAAAACCACCCCA

PARP1

CGCCTGTCCAAGAAGATGGT

AAGGCACTTGCTGCTTGTTG

PARP2

AGCAAGATGAATCTGTGAAGGC

AGGCTGTGCTGTCCCATTTT

BRCA1

AATTGCGGGAGGAAAATGGG

CTTCACCACAGAAGCACCAC

BRCA2

GAAGCGTGAGGGGACAGATT

GATTGGTACAGCGGCAGAGT

Rad51

CTACTGGCTCCAAAGAGCTTGA

ACCACTGCTACACCAAACTCATC

Rad52

CCGAGGCGCAGGTCAAC

ATCCACATTCTGCTGCGTGA

c-Myc

TTTGCACTGGAACTTACAACACC

CCTCCTCGTCGCAGTAGAAAT

NFкB

ACTACGAGGGACCAGCCAAGA

CGCAGCCGCACTATACTCA

PIK3CA

CCACGCAGGACTGAGTAACA

CCAAGCACCGAACAGCAAAA

Akt

CTCTTTCCAGACCCACGACC

TAATGTGCCCGTCCTTGTCC

FOXO1

ACAGCCCTGGATCACAGTTT

TTTGGTAGTTTGGGCTGGGT

FoxO3a

GAGAGCTCCCCGGACAAAC

CTGTCGTCAGCTGATTCGGG

FOXM1

GAGCAGCGACAGGTTAAGGT

TGTGATTCCAAGTGCTCGGG

Exo1

CGGGCCAACAATACCTTCCT

TCTTCTGACAGCTCTGCACT

β-Actin

GACCTGACTGACTACCTC

TCTTCATTGTGCTGGGTGC

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