Figure 3

(A) Hippocampal panoramic view taken from a Cyp27Tg mouse intracellularly injected in CA1 with LY (green) and stained with DAPI (blue). (B) Higher magnification of A showing single CA1 pyramidal neurons individually injected with LY. (C−J) Representative pictures (63X oil; pixel size 0.057 × 0.057; z-step 0.14) of basal (Or; stratum oriens) dendrites (C−F) and apical (Rad; stratum radiatum) dendrites (G−J) from WT (C, E, G, I) and Cyp27Tg (D, F, H, J) mice. (K−N) 3D reconstruction of the complete spine morphology. (K) Dendritic segment from a CA1 pyramidal neuron in the stratum oriens from a WT mouse. For each stack of images, a solid surface was created to match the dendritic shaft (L). Various intensity threshold surfaces were then created (M), and the solid surface that exactly matched the contours of the dendritic spine volume was then selected for each dendritic spine (N). Scale bar shown in N indicates 300 µm in A, 40 µm in B, 10 µm in C−J and 5 µm in K−N.