Figure 5
From: High levels of 27-hydroxycholesterol results in synaptic plasticity alterations in the hippocampus
Cyp27Tg mice showed increased numbers of synaptopodin-ir puncta in the stratum radiatum. (A) Panoramic view of the hippocampus from a WT mouse stained with anti-synaptopodin antibody. (B, C) Representative pictures of the CA1 region in WT (B) and Cyp27Tg (C) mice, respectively. White squares represent the regions analyzed with confocal microscopy. Two different distances from the pyramidal layer were analyzed: 20−80 and 60−120 μm (distal stratum radiatum). (D) Photomicrograph in CA1 region from a WT mouse showing the synaptopodin-ir puncta distribution. (E−F) Comparative analysis showing scatter plots (GraphPad Prism 5.0) of the number of synaptopodin-ir puncta/ μm3 for the (E) stratum radiatum and (F) distal stratum radiatum for Cyp27Tg compared to WT mice. Unpaired Mann–Whitney test was employed to compare groups. s.r., stratum radiatum; s.o., stratum oriens; p.l., pyramidal layer. All data are represented as mean ± SEM. Scale bar shown in A indicates 500 μm in A, 100 μm in B and C, and 10 μm in D.
