Figure 2

Diagrams of the segments from the COI and Tpi genes used for the genetic analysis (derived from42). (A) COIB gene segment identifying PCR primers used to amplify the fragment (block arrows) and the strain and COI-h haplotype defining the mCOI1164D and mCOI1287R polymorphic sites. CO1-RS is defined as a T and A at mCOI1164D and mCOI1287R, respectively. There are four corn-strain (CO1-CS) haplotypes (h1–h4) with an A or G observed at both mCOI1164D and mCOI1287R. (B) Portion of the fall armyworm Tpi gene with block arrows indicating PCR primers. The TpiE4 exon segment contains the gTpi183 site that is polymorphic for a C or T. Representative DNA chromatograph patterns are shown to illustrate how TpiC, TpiR, and TpiH are defined. The TpiI4 intron segment lies adjacent.