Figure 1 | Scientific Reports

Figure 1

From: Using antisense oligonucleotides for the physiological modulation of the alternative splicing of NF1 exon 23a during PC12 neuronal differentiation

Figure 1

Analysis of the alternative splicing of E23a of the NF1 gene in different cellular models. (A) Top: Phase-contrast images of PC12 cells without (left) or with (right) NGF. Bottom: Schematic representation of E23a alternative splicing during PC12 neuronal differentiation and RT-PCR time course analysis of the switch from Nf1 type II (+E23a) to type I (-E23a) transcripts after NGF addition to PC12 cells. (B) Top: Phase contrast images of H19-7/IGF-IR cells without (left) or with (right) differentiation conditions. Bottom: RT-PCR analysis of Nf1 type II (+E23a) switch to type I (-E23a) transcript at 24 h is shown. (C) Top: Phase contrast images of proliferating nerve-derived primary Schwann cells (left) and differentiating (right) conditions. Bottom: RT-PCR analysis of NF1 isoform switch is illustrated. For all phase-contrast images scale bar is 30 μm. E23a splicing was quantified as the percent spliced-in (PSI) (standard deviation in parentheses). All supporting uncropped gels can be found in Supplementary Fig. S1.

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