Figure 1 | Scientific Reports

Figure 1

From: (E)-N-(2-(3, 5-Dimethoxystyryl) phenyl) furan-2-carboxamide (BK3C231) induces cytoprotection in CCD18-Co human colon fibroblast cells through Nrf2/ARE pathway activation

Figure 1

Effect of BK3C231 pretreatment on the activity of cytoprotective enzymes namely NQO1 (a), GST (b), and HO-1 (c). The enzymatic activity of cytoprotective enzymes was observed at basal level in untreated control cells (CON) and cells treated only with BK3C231 (BK3C231 24 h). However, in cells treated only with 4NQO (4NQO 1 h), there was a decrease in the activity of cytoprotective enzymes as compared to that of untreated control cells. Pretreatment of cells with BK3C231 before 4NQO exposure increased the activity of cytoprotective enzymes, significantly at 24 h as compared to that of cells treated with 4NQO only. Treated whole cell lysates were subjected to spectrophotometric and microplate reader data analysis as mentioned in “Results” to determine the activity of NQO1, GST and HO-1. Each data point was expressed as mean ± SEM from at least three independent experimental replicates. * p < 0.05 against 4NQO 1 h.

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