Figure 3 | Scientific Reports

Figure 3

From: Evaluation of the ex vivo liver viability using a nuclear magnetic resonance relaxation time-based assay in a porcine machine perfusion model

Figure 3

Detection of biliary duct injury (BDI) in different degree of warm ischemia injury. (A) Histological images of the bile duct with indicated WIT (0′, 30′, 60′). Scale bar = 100 μm. (B) Evaluation of BDI score of the bile duct with indicated WIT. (data represent mean ± SD. Student’ s t-test was used for analyzing between groups.*P < 0.05, **P < 0.01). (C) The evaluation of biliary pH of livers with different WIT (0′, 30′, 60′). Bile samples were collected at indicated time points after NMP. Biliary pH was tested at different time points after NMP initiation (data represent mean ± SD. Two-way ANOVA was used for analyzing the differences among three groups. Group a (0′) vs. Group c(60′): *P < 0.05, **P < 0.01;Group b (30′) vs. Group c (60′): #P < 0.05, ##P < 0.01). (D) The evaluation of biliary bicarbonate of livers with different WIT (0′, 30′, 60′). Bile samples were collected at indicated time points after NMP. Biliary bicarbonate was tested at different time points after NMP initiation (data represent mean ± SD. Two-way ANOVA was used for analyzing the differences among three groups. Group a (0′) vs. Group c (60′): *P < 0.05, **P < 0.01; Group b (30′) vs. Group c (60′):#P < 0.05, ##P < 0.01).

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