Figure 6
From: MTBP phosphorylation controls DNA replication origin firing
Phosphorylation of MTBP on CDK consensus sites promotes origin firing in unperturbed growth conditions. (A,B) Quantification of replication activity (A) or cell cycle distribution (B) using BrdU-flow cytometry as described in 2D/E of indicated HeLa Flip-In T-Rex cell lines. Error bars, SEM. n = 3; MTBP mutants: MTBP-6A/D, see 5C. Significance tests as in 2D/E. (C) Whole cell lysates of stable cell lines described in A were analysed by immunoblotting using anti-MTBP (12H7), and Ponceau (Pon.) staining. (D) Inter-origin distance of cells as described in A measured by DNA combing as described in 4C. See also Supplementary Information Fig. S10.
