Figure 2 | Scientific Reports

Figure 2

From: Disturbance of cellular homeostasis as a molecular risk evaluation of human endothelial cells exposed to nanoparticles

Figure 2

Comparisons of NP cytotoxicity in human endothelial cell line. (A,B) HUVEC-ST cell viability measured by the neutral red assay (left panels) or Alamar Blue assay (resazurin oxidation; right panels) after cell incubation for 72 h with increasing concentrations of NPs. Values are the mean ± SD of three independent experiments with six replicates in each experiment. Statistically significant differences comparing control cells taken as 100%, *p < 0.05 (C) Light microscopy images under contrast phase conditions of HUVEC-STs after treatment for 24 h with 100 μg/mL of PLA/MMT/TRA, PLA/EDTMP, PLGA/MDP, or 0.025 μg/mL of Pluronic F127 Ms. Bar = 50 µm. (D,E) Growth kinetics of HUVEC-STs treated with PLA/MMT/TRA, PLA/EDTMP, PLGA/MDP [100 μg/mL (D) or 250 μg/mL (E)], or Pluronic F127 Ms [0.25 μg/mL (D) or 0.5 μg/mL (E) for 72 h. ***p < 0.001, values are % of those obtained for untreated control (mean ± SD; n ≥ 3).

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