Table 2 Primer sequences used for RT-PCR.
Gene | Strand | Sequence 5′–> 3′ |
|---|---|---|
Hypoxanthine–guanine phosphoribosyltransferase (HPRT1) | Forward | TGACACTGGCAAAACAATGCA |
Reverse | GGTCCTTTTCACCAGCAAGCT | |
Bcl2-like protein 4 (Bax) | Forward | GTTTCATCCAGGATCGAGCAG |
Reverse | CATCTTCTTCCAGATGGTGA | |
B-cell lymphoma 2 (Bcl2) | Forward | AGGAAGTGAACATTTCGGTGAC |
Reverse | GCTCAGTTCCAGGACCAGG | |
Cytochrome complex (Cyt c) | Forward | AGGCCCCTGGATACTCTTACACAG |
Reverse | TCAGTGTATCCTCTCCCCAGATG | |
H2A histone family member X (H2AX) | Forward | ACGAGGAGCTCAACAAGCT |
Reverse | GTGGCGCTG GTCTTCTTG | |
ATM serine/threonine kinase (ATM) | Forward | GCAGCTGGAAGAAGCACAA |
Reverse | TTTTAGGCTGGGATTGTTCG | |
Ataxia telangiectasia and Rad3-related protein (ATR) | Forward | TGTAGAGAGATGGAGACCAACG |
Reverse | GACCAATCGGTTGACTTCTGA | |
Poly (ADP-ribose) polymerase (PARP) | Forward | GTGTGGGAAGACCAAAGGAA |
Reverse | TTCAAGAGCTCCCATGTTCA | |
Cytochrome c (Cyt c) | Forward | AGGCCCCTGGATACTCTTACACAG |
Reverse | TCAGTGTATCCTCTCCCCAGATG | |
Apoptosis inducing factor (AIF) | Forward | ATGGAGTTTGACCTTAATGG |
Reverse | GCAACTCAGAGATAGCTTTG |
