Figure 1

Validation and characterization of isolated EV: Size and concentration distribution of nanoparticles from NTA in triplicate (yellow, red and orange graphs) (A); ds-DNA antibody labelled TEM image at magnification × 40,000 (B); and Western blots using common exosome markers (positive for CD9 and negative marker Calnexin) in melanoma cell lysate (SKMel28) as positive control (lane 10) and isolated EV (lane 7) (C). Lanes 2, 3, 4, 5, 6, 8, 9 were of other samples that were included in the same Western Blot analysis, but were not patients included in the study.