Figure 5
From: Life-long brain compensatory responses to galactic cosmic radiation exposure
Enhancements in Schaffer collateral-CA1 LTP and spatial learning 20 months after exposure to 56Fe particle radiation (a) Time course and magnitude (inset bar graph) of stimulus-evoked LTP in slices from male mice exposed to 100 cGy radiation, compared to sham-irradiated controls (0 cGy). After a 15 min baseline, LTP was elicited by two TBS stimulus trains (arrows), and the magnitude of LTP between 35–40 min post TBS or bath application (perforated box) compared across doses. *P < 0.0001, Two-tailed t test, n = 12–16 slices per dose. (b) Time course and magnitude (inset bar graph) of chemically-evoked LTP elicited by bath application (bar) of 10 µM forskolin plus 10 µM rolipram to slices from male mice exposed to 100 cGy radiation compared to sham-irradiated controls (0 cGy). Each point represents mean ± SEM. *P < 0.0001, Two-tailed t test, n = 12–16 slices per dose. (c, d) Learning curves depicting (c) latency for entry into an escape box in the Barnes Maze, Two-way RM ANOVA, dose: F(1,90) = 30.73, P < 0.0001; trial: F(4,90) = 2.22, P = 0.073; interaction: F(4,90) = 0.553, P = 0.698, Tukey’s post hoc test: Day 2, 0 cGy vs. 100 cGy, P = 0.006, Day 4, 0 cGy vs. 100 cGy, P = 0.027, or (d) the fraction of successful trials per day, Two-way RM ANOVA, dose: F(1,90) = 10.51, P = 0.002, trial: F(4,90) = 3.06, P = 0.020; interaction: F(4,90) = 0.849, P = 0.497, (Tukey’s post hoc test did not reveal significant within treatment effect), are shown for male mice exposed to 100 cGy radiation compared to sham-irradiated controls (0 cGy). Each point represents mean latency ± sem. *P < 0.05, n = 10 mice per dose.
