Figure 12

Influence of lipoproteins on the filtration rate of endotoxin. 5 ng/mL LPS (E. coli) were dissolved in PBS, lipoprotein-depleted serum, native serum and lipoprotein-enriched serum and filtered through spin columns with 100, 300 and 1000 kDa MWCO. Additionally, the samples were filtered through a 0.1 µm and 0.22 µm sterile filter. The figure shows the percentage of endotoxin values measured in the filtrate samples compared to the unfiltered samples (N = 3).