Figure 7

Endotoxin activity in serum (A), 4% HSA solution (B) and aqueous protein-free saline solution (C) as a function of divalent cation levels. The three media were spiked with increasing concentrations of divalent cations (iCa, iMg or iCa/iMg in 2:1 ratio). The x-axis shows the sum of iCa + iMg value in mM. Afterwards, the recovery of 5 ng/mL LPS (E. coli) was determined by LAL test. Mean ± standard deviation of three independent experiments are shown.