Figure 8
Endotoxin neutralization as a function of incubation time in different media. To check whether endotoxin neutralization in different media is time-dependent, 5 ng/mL LPS (E. coli) were incubated in different solutions for 24 h (reverse mode) and the endotoxin recovery was measured by LAL test. The kinetic LPS neutralization was tested in ringer solution and ringer solution containing 4% (w/v) human serum albumin (A). Fresh human serum, heparin plasma and lipoprotein-enriched serum were also included in this study (B). The bars marked with * indicate from which incubation time of LPS a significant (p ≤ 0.05) endotoxin reduction is achieved compared to the 10 min sample (C and D). n.s. not significant. Mean ± standard deviation of three independent experiments are shown.
