Figure 9
Influence of citrate anticoagulation on endotoxin activity in human plasma. To check whether the time-dependent endotoxin neutralization of 5 ng/mL LPS (E.coli) is influenced by citrate anticoagulation, LPS was incubated in different plasma and serum samples for 24 h (reverse mode) and the endotoxin recovery was measured by LAL test (A). The kinetic LPS neutralization was tested in citrate plasma (citrate plasma-LPS), in serum and in serum where citrate was added after the incubation period immediately before LAL analysis (serum-LPS-citrate). The bars marked with * indicate from which incubation time of LPS a significant (p ≤ 0.05) endotoxin reduction is achieved compared to the 10 min sample (B). n.s. not significant. Mean ± standard deviation of three independent experiments with plasma/serum from different donors are shown.
