Figure 10 | Scientific Reports

Figure 10

From: The PHP domain of PolX from Staphylococcus aureus aids high fidelity DNA synthesis through the removal of misincorporated deoxyribo-, ribo- and oxidized nucleotides

Figure 10

Proofreading activity of wt-saPolX. Time-course analysis of 3′–5′ exonuclease activity exhibited on 1 nucleotide gapped DNA substrates bearing normal (C:G) and mismatched (T:G) base pairs in the presence of Mn2+ by saPolX wt (12.5 nM) (A,B) and H435A (12.5 nM) (C,D), respectively. LC represents the loading control (6FAM labelled 34mer DNA oligonucleotide) added in each lane for quantification. The time course of exonuclease activity of wt-saPolX and H435A on matched and mismatched gapped DNA are displayed (E). The exonuclease activity of wt-saPolX and H435A at 120 min incubation was also compared (F). saPolX showed higher exonuclease activity when it encountered a mismatched base pair as compared to the correctly paired Watson–Crick base pair.

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