Figure 11 | Scientific Reports

Figure 11

From: The PHP domain of PolX from Staphylococcus aureus aids high fidelity DNA synthesis through the removal of misincorporated deoxyribo-, ribo- and oxidized nucleotides

Figure 11

Exonuclease activity of wt-saPolX to remove ribonucleotides. Time-dependent 3′–5′ exonuclease activity exhibited on gapped DNA containing rUMP incorporated against templating A and C nucleotide in the presence of divalent metal ion Mn2+ by wt-saPolX (A,B) and H435A mutant (50 nM each) (C,D), respectively. LC represents the loading control added in each lane for quantification. In graph (E), the time course analysis of wt-saPolX and H435A for ribonucleotide excision is displayed. In bar graph (F), the level of ribonucleotide excision by wt-saPolX and H435A for 120 min incubation is compared.

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