Figure 3 | Scientific Reports

Figure 3

From: The PHP domain of PolX from Staphylococcus aureus aids high fidelity DNA synthesis through the removal of misincorporated deoxyribo-, ribo- and oxidized nucleotides

Figure 3

Purification of wt-saPolX and H435A protein. (A) The gel filtration chromatogram for wt-saPolX is shown. The gel filtration profile showed the presence of a single peak with VR ~ 74 ml. (B) SDS-PAGE analysis of purified wt-saPolX protein showed the presence of a single band corresponding to 66.97 kDa. The H435A and H435A/D193A mutants used in this study were purified with the same protocol as wt-saPolX. The gel filtration chromatogram for mutant proteins also showed the presence of a single peak with VR ~ 74 ml and SDS-PAGE analysis of purified mutants showed the presence of a single band in gel.

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