Figure 4 | Scientific Reports

Figure 4

From: The PHP domain of PolX from Staphylococcus aureus aids high fidelity DNA synthesis through the removal of misincorporated deoxyribo-, ribo- and oxidized nucleotides

Figure 4

Enzyme activities of wt-saPolX, mutant H435A and double mutant H435A + D193A. Primer extension assays were performed on a gapped DNA substrate to check the polymerase and exonuclease activities present in wt-saPolX and exonuclease mutant H435A. The results show that wt-saPolX possesses both polymerase and exonuclease activities. The mutation H435A abolished the exonuclease activity of saPolX and the mutation D193A removed the polymerase activity.

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