Figure 6 | Scientific Reports

Figure 6

From: The PHP domain of PolX from Staphylococcus aureus aids high fidelity DNA synthesis through the removal of misincorporated deoxyribo-, ribo- and oxidized nucleotides

Figure 6

Fidelity of DNA synthesis by saPolX. Primer extension assays were conducted to assess the ability of saPolX to incorporate dATP, dTTP, dGTP and dCTP opposite the four possible template nucleotides- dA, dT, dG and dC. The identity of the template nucleotide is mentioned at the top of each gel. wt-saPolX shows incorporation according to normal Watson–Crick base pairing (A–D). H435A mutant shows accurate incorporation opposite templating bases A, G and C (E–H) but exhibits significant misincorporation of dGTP opposite template dT (F).

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