Figure 7 | Scientific Reports

Figure 7

From: The PHP domain of PolX from Staphylococcus aureus aids high fidelity DNA synthesis through the removal of misincorporated deoxyribo-, ribo- and oxidized nucleotides

Figure 7

Comparison of rNTP incorporation by wt-saPolX and H435A. Primer extension assays were conducted to assess the ability of saPolX to incorporate rATP, rUTP, rGTP and rCTP opposite the four possible template nucleotides- dA, dT, dG and dC. The identity of the template nucleotide is mentioned at the top of each gel picture. wt-saPolX does not incorporate any rNTP as shown in panels (A–D), respectively. saPolX-H435A shows incorporation of (F) rATP opposite dT, (G) rCTP opposite dG and (H) rGTP opposite dC (E) except incorporation opposite dA.

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