Figure 8 | Scientific Reports

Figure 8

From: The PHP domain of PolX from Staphylococcus aureus aids high fidelity DNA synthesis through the removal of misincorporated deoxyribo-, ribo- and oxidized nucleotides

Figure 8

Comparison of incorporation of oxidized nucleotides by wt and mutant versions of saPolX. The incorporation of 8oxodGTP (oG) opposite all four possible templating deoxynucleotides was compared to the incorporation of dGTP opposite dC for (A) wt-saPolX and (B) mutant H435A. Whereas wt-saPolX does not incorporate 8oxodGTP at all, H435A incorporates 8oxodGTP preferentially opposite template dA instead of dC. The incorporation of oxidized nucleotide 2-OH-dATP (oA) opposite all four possible templating deoxynucleotides was compared to that of incorporation of dGTP opposite dC for (C) wt-saPolX and (D) mutant H435A. The native enzyme incorporated 2-OH-dATP opposite dTTP but the mutant version misincorporated the damaged nucleotide opposite template dG and dC also.

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