Table 2 Oligonucleotides used in this study are mentioned below in the table.

From: The PHP domain of PolX from Staphylococcus aureus aids high fidelity DNA synthesis through the removal of misincorporated deoxyribo-, ribo- and oxidized nucleotides

Name (size)

Oligonucleotides

T1 (50mer)

5′TCCTACCGTGCCTACCTGAACAGCTGGTCACATAAATGCCTACGAGTACG3′

T2 (50mer)

5′TCCTACCGTGCCTACCTGAACAGCTGGTCACATATATGCCTACGAGTACG3′

T3 (50mer)

5′TCCTACCGTGCCTACCTGAACAGCTGGTCACATAGATGCCTACGAGTACG3′

T4 (50mer)

5′TCCTACCGTGCCTACCTGAACAGCTGGTCACATACATGCCTACGAGTACG3′

40A (40mer)

5′TAATCATAAGTATCAGGACTCTCTCTCTCTCAACGGGGGG3′

40C (40mer)

5′TAATCATAAGTATCCGGACTCTCTCTCTCTCAACGGGGGG3′

26 T (26mer)

5′CGGACTCTCTCTCTCTCAACGGGGGG3′

P1 (15mer)

5′XCGTACTCGTAGGCAT3′

P2 (34mer)

5′YTATGTGACCAGCTGTTCAGGTAGGCACGGTAGGA3′

26P (26mer)

5′XCCCCCCGTTGAGAGAGAGAGAGTCCG3′

13P (13mer)

5′YATACTTATGATTA3′

T5 (51mer)

5′TCCTACCGTGCCTACCTGAACAGCTGGTCACATATTATGCCTACGAGTACG3′

T6 (51mer)

5′TCCTACCGTGCCTACCTGAACAGCTGGTCACATATCATGCCTACGAGTACG3′

T7 (51mer)

5′TCCTACCGTGCCTACCTGAACAGCTGGTCACATATAATGCCTACGAGTACG3′

P3 (16mer)

5′XCGTACTCGTAGGCsAsTG3′

P4 (16mer)

5′XCGTACTCGTAGGCsAsTZ3′

P5 (26mer)

5′XCCCCCCGTTGAGAGAGAGAGAGTCsCU3′

LC (34mer)

5′XCGTACTCGTAGGCAGTCGCGAGACCAGCTGTTCG3′

  1. The primers P3, P4, and P5 have phosphorothioate linkages between the mentioned nucleotides at 3′ end which are represented by subscript “s”. X, Y, and Z represent 6FAM, 5′phosphate group, and 8oxodG, respectively.
Back to article page