Figure 3 | Scientific Reports

Figure 3

From: Impact of cigarette versus electronic cigarette aerosol conditioned media on aortic endothelial cells in a microfluidic cardiovascular model

Figure 3

Analysis of ICAM-1 and E-selectin expression in HAECs following 24 h exposure to WSCM. (a) Immunofluorescence microscopy analysis and associated quantification of ICAM-1 or (b) E-selectin expression following HAEC exposure to WSCM for 24 h in Ibidi chamber slides. Images were captured using a Zeiss Axio Observer.Z1 microscope (×20 magnification). Scale bar (yellow line) = 70 µm. Errors bars denote ± SD (n = 6). (c) Western blot analysis and associated quantification of ICAM-1, VCAM-1 and E-selectin expression in HAECs following 24 h exposure to WSCM (refer to Supplementary Figure S1 for uncropped blots). One-way ANOVA with Dunnett’s multiple comparisons between vehicle control and each WSCM concentration. Error bars denote ± SD (n = 3); *p < 0.0 **p < 0.01 ***p < 0.001.

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