Figure 6 | Scientific Reports

Figure 6

From: Impact of cigarette versus electronic cigarette aerosol conditioned media on aortic endothelial cells in a microfluidic cardiovascular model

Figure 6

Analysis of ICAM-1 and CD11b co-localisation in HAECs following 24 h exposure to test article in the BioFlux microfluidic system. Immunofluorescence microscopy analysis of ICAM-1 and CD11b/MAC-1 co-localisation between monocytes and HAECs following 24 h WSCM, eVCM or nicotine exposure in BioFlux microfluidic channels. Monocyte adhesion to HAECs was assessed over a course of adhesion periods (10, 20, 30 and 40 min). Images were captured after the 40 min adhesion period using a Zeiss LSM780 confocal microscope (×40 magnification). Scale bar (yellow line) = 35 µm. To assess co-localisation, z-planes were applied through the composite z-stack. Inset images represent z-planes from regions marked with a white line.

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