Figure 8 | Scientific Reports

Figure 8

From: Impact of cigarette versus electronic cigarette aerosol conditioned media on aortic endothelial cells in a microfluidic cardiovascular model

Figure 8

Analysis of ICAM-1 involvement in test article-induced monocyte adhesion to HAECs in the BioFlux microfluidic system. Quantification of monocyte adhesion to HAECs over adhesion periods of 10, 20, 30 and 40 min following 72 h transfection with control or ICAM-1 siRNA and 24 h HAEC exposure to (a) nicotine, (b) WSCM or (c) eVCM, with fold change graphs inset and representative brightfield images of BioFlux microfluidic channels captured after the 40 min adhesion period using a Zeiss Axio Observer.Z1 microscope (×10 magnification). Two-way ANOVA with Dunnett’s multiple comparisons between all treatment conditions. Error bars denote ± SD (n = 6); *p < 0.05 **p < 0.01 ***p < 0.001.

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