Figure 2
Expression and localization of Pfα-tubulin-I and Pfβ-tubulin in parasite with respect to apicortin along with localization of apicortin in subcellular fractions. (A) Expression of PfApicortin(green channel) and Pfα-tubulin-I(red channel) in mature schizont and merozoites along with colocalization of it with PfApicortin (merged image and graph), (B) Expression of PfApicortin (green channel) and Pfβ-tubulin (red channel) in mature schizont along with localization of it with PfApicortin (merged image and graph), scale bar represents distance of 5 µm. Images were analyzed with Cellsense Dimension 3 (https://www.olympus-lifescience.com/en/software/cellsens/) and ImageJ (imagej.nih.gov) softwares, (C) Blot showing bands of apicortin in the cytoplasmic and nuclear fractions along with the gel showing the loading control (Supplementary Fig. S9A,B) and the blots indicating the level of H4(histone) and PfNapL in nuclear and cytoplasmic fractions as controls (Supplementary Fig. S9C,D).
