Figure 3

Phenotypic characterization of VDOP cells. Localization of various endothelial markers lectin, VEGFR2, CD34 and NG2 in NTM (a–d), SC (e–h) and VDOP (i–l) cells using immunohistochemistry. All three cell types were positive for Lectin (a, e, i) while VEGFR2 was seen only in SC and VDOP cells (f, j). CD34 was found only in SC cells (g) whereas both NTM and SC cells stained positive for NG2 (d, h). Based on this staining pattern, NTM cells were defined as Lectin⁺VEGFR2^-CD34^-NG2⁺, SC cells as Lectin⁺VEGFR2⁺CD34⁺NG2⁺ and VDOP cells as Lectin⁺VEGFR2^-CD34^-NG2^-.A total of 8 VDOP (6 at passage 4 and 2 at passage 5), 3 NTM (2 at passage 5, one at passage 6) and 3 SC (all at passage 6) cell lines were used for this experiment. Scale bar, 20 µm.