Figure 4

The effect of ACC deaminase on shoot element content in C. sativa grown in the absence or presence of salt. Soil was treated with buffer (control) or P. migulae 8R6 producing ACC deaminase. Transgenic lines tested were independent, single insert, homozygous lines expressing the Pseudomonas sp. UW4 acdS gene under the control of either the rolD or the CaMV 35S promoter. Panels show macro- and micro-element content in shoots of plants 33 days after sowing that were irrigated with either tap water or with a salt solution (15 or 20 dS m−1); application of the salt solution began 20 days after sowing. Significant differences between groups were detected using a two-way ANOVA and Tukey post-test. Values that are significantly different (p < 0.05) from the control are indicated by asterisks (*). Error bars indicate standard error (n = 3 biological replicates). Plots were drawn using SigmaPlot ver. 13.0 (Systat Software, Inc., San Jose, USA, www.systatsoftware.com).