Figure 5
A metabolic switch occurs along tumor stiffening, in Mesenchymal HGSOC. (A–F) Representative pathways up-regulated in different tumor stiffness conditions and tumor localizations, as indicated by the different schemes. A plain circle (epithelial compartment) surrounded by wavy lines (stromal compartment) represents soft tumors. A plain circle (epithelial compartment) surrounded by straight lines (stromal compartment) illustrating matrix contraction, represents the core of stiff tumors. A thick circle line (epithelium) surrounded by straight lines (stroma) represents the periphery of stiff tumors. Orange represents the stromal compartment localization and green the epithelial compartment localization when performing differential analysis in stiff vs. soft tumors or within stiff tumors. Stromal and epithelial genes expression are based on murine MTA 1.0 or human HTA 2.0 microarrays, respectively. T-test was used to compare gene expression between two conditions. For each condition, the top 3 DAVID biological pathways defined by the up-regulated gene lists are represented. We analyzed 3 soft tumors, 5 stiff tumors both at their periphery or at their center. P values are presented as – Log 10. (A) Genes up-regulated in the epithelium at the center of stiff tumors (left) compared to genes up-regulated in the epithelium of soft tumors (right). (B) Genes up-regulated in the epithelium at the periphery of stiff tumors (left) compared to genes up-regulated in the epithelium of soft tumors (right). (C) Genes up-regulated in the epithelium at the center (left) compared to the periphery (right) of stiff tumors. (D) Genes up-regulated in the stroma at the center of stiff tumors (left) compared to genes up-regulated in the stroma of soft tumors (right). (E) Genes up-regulated in the stroma at the periphery of stiff tumors (left) compared to genes up-regulated in the stroma of soft tumors (right). (F) Genes up-regulated in the stroma at the center (left) compared to the periphery (right) of stiff tumors.
