Figure 6
From: Inverse relation between structural flexibility and IgE reactivity of Cor a 1 hazelnut allergens
Specific IgE-binding to the four hazelnut isoforms Cor a 1.0401 (blue), Cor a 1.0402 (light blue), Cor a 1.0403 (yellow), and Cor a 1.0404 (orange). (a) Bar plots and standard deviations of IgE-binding potentials obtained by ELISA for each patient (n = 22), using identical amounts of recombinantly produced allergen. (b) Box plots of the IgE-binding potentials of the four isoforms. The boxes are restricted by the 25th and 75th percentile, median values are given by a horizontal line inside the box and whiskers cover the minimal and maximal values inside the interquartile range (1.5 IQR). Patients with high total IgE levels and therefore high specific IgE are indicated by dots. (c) ELISA data (median values) and ImmunoCAP data (black). Differences between the IgE-binding potentials are given by the lines above with a p value < 0.05 as statistically significant. The exact pairwise p values are 0.006 (01–02), 0.453 (01–03), 1.89 × 10–8 (01–04), 0.565 (01-ImmunoCAP), 1.000 (02–03), 0.100 (02–04), 9.33 × 10–7 (02-ImmunoCAP), 0.001 (03–04), 0.001 (03-ImmunoCAP), and 2.44 × 10–14 (04-ImmunoCAP). All three panels suggest the immunologic ranking Cor a 1.0401 > 03 > 02 > 04.
