Figure 2

Mapping minimal sequences in HS1 Fab-6 required for boundary function. (a) Schematic diagram of the Fab-6 replacements. The coordinates of the Fab-6 fragments (F6′) are according to the BX-C sequence61. On the right side: scheme of the replacements after integration in F61attP platform. The mCherry and mini-y genes are indicated by magenta and yellow arrows respectively. (b) Morphology of the male abdominal segments (numbered) in F61attP, F61330, F6873 and F6529. The filled red arrowheads show morphological features indicative of GOF transformations. The empty red arrowheads show LOF transformations. Black arrowheads indicate pigmented spots that are the result of ectopic activation of the mini-y reporter. All other designations are the same as described in Fig. 1.