Figure 1 | Scientific Reports

Figure 1

From: 3D spheroid models of paediatric SHH medulloblastoma mimic tumour biology, drug response and metastatic dissemination

Figure 1

Schematic diagram describing the establishment of the 3D spheroid model and its applications. On day 0, cells are harvested and seeded into 96-well ultra-low attachment, round-bottom plates at an optimal cell density to achieve 3D spheroids with a diameter of 250–350 µm by day 4. 3D spheroids can then be used in numerous functional assays, including growth and drug response analysis, as well as migration and invasion assays using matrices.

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