Figure 2
Growth characterisation of SHH medulloblastoma 3D spheroids. (a) Growth of SHH medulloblastoma 3D spheroids seeded at their optimal cell density in either neurosphere (red) or standard (blue) medium was assessed over a seven-day period. Spheroid diameter (µm) was significantly enhanced in neurosphere medium for all cell lines. Error bars represent the mean ± SEM of n = 3 experiments each containing 3 replicates. Significance was calculated using two-way ANOVA analyses with Sidak’s multiple comparisons post-hoc test (*p ≤ 0.05, ***p ≤ 0.001, ****p ≤ 0.0001). (b) Scatter plots displaying little variation in 3D spheroid diameter on day 4 over three independent experimental repeats (n = 1, 2, and 3) for the SHH medulloblastoma cell lines DAOY, ONS76, and UW228-3. Error bars represent the mean ± SD across plates containing 20–24 3D spheroids. (c) Growth analysis of 3D spheroids up to 21 days in culture. Relationship between 3D spheroid volume (µm3) and time was assessed using Pearson’s correlation coefficient (r). Error bars represent the mean ± SEM (n = 4) containing 6 replicates at each time point. (d) Representative images of 3D spheroids at each time point are shown (scale bar 100 µm).
